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Laboratory | Structural biology | Radiobiology


LBSR

Laboratory of Structural Biology and Radiobiology

The Laboratory of Structural Biology and Radiobiology studies the 3D structure of proteins involved in the structural organization and genomic integrity of the cell nucleus. The various topics developed around this theme have the common aim of describing protein/protein interaction networks involved in biological functions essential for cell survival such as signaling and repair of DNA damages. The target proteins are modular: they include well-folded fragments connected by flexible segments.

 

​​FRENCH

 

Published on 27 June 2018


Team Leader
Jean-Baptiste CHARBONNIER
Tél : 01 69 08 76 77
JB.CHARBONNIER@cea.fr 

Nuclear envelope, telomeres and DNA repair 
(Jean-Baptiste CHARBONNIER, Marie-Hélène LE DU, Sophie ZINN-JUSTIN)
Molecular assemblies and genome integrity 
(Raphaël GUEROIS & Françoise OCHSENBEIN)

Staff
Jessica Andreani, Researcher
Yves Boulard, Researcher
Jean-Baptiste Charbonnier, Researcher
Stéphane Bressanelli, Researcher
Philippe Cuniasse, Researcher
Pascal Drevet, Researcher
Sonia Fieulaine, Researcher
Bernard Gilquin, Researcher
Raphaël Guerois, Researcher
Marie-Helene Le Du, Researcher
Michel Masella, Researcher
Simona Miron, Researcher
G wenaëlle Moal-Raisin, Technician
Françoise Ochsenbein, Researcher
Maïté Paternostre, Researcher
Laure Plançon-Arnould, Univ. PSud Lecturer
Carine Tellier-Lebegue, Technician
François-Xavier Theiller, Researcher
Sophie Zinn-Justin , Researcher    

 

Crystallography

The globular modules or domains have multiple functions including regulation of the enzymatic activities of proteins and orchestration of the cellular signaling. They are usually conserved during evolution, and we identify them using bioinformatics approaches. Their 3D structure is determined by NMR or X-ray crystallography. Their arrangement within the modular protein is observed by SAXS and NMR. Their interactions are characterized by NMR, biochemistry, fluorescence or calorimetry. Crystallography provides access to the structure of multi-functional protein complexes. Our structures are positionned in electron microscopy maps in order to build pseudo-atomic models of functionally important complexes.

Molecular modeling

Clearly, molecular modeling is a central tool in the laboratory that gives access to models consistent with heterogeneous structural data. On the experimental point of view, in addition to standard laboratory equipment, we have a comprehensive NMR park (500MHz, 600MHz, 700MHz), and we benefit from the proximity of the synchrotron SOLEIL which is in operation since 2006. The laboratory has an extensive expertise in the different techniques that give access to an atomic understanding of biological processes. Its location in an environment of cell biologists and geneticists favors synergies between in vitro and in vivo approaches.