Frédéric Joliot Institute for Life Sciences - On the same theme https://joliot.cea.fr/drf/joliot/en/Pages/news/Science/2020/Salmonella-shigella-cross-protection.aspx Website of the Frédéric-Joliot Institute of Life Sciences which includes 4 departments: I2BC, DMTS, NeuroSpin and Service hospitalier Frédéric Joliot (SHFJ). The teams are mainly located at the CEA Paris-Saclay center and at the Orsay hospital. en-US https://www.cea.frhttps://www-cea-fr.admsite.extra.cea.fr/Style%20Library/Themes/drf/instituts/images/logo-cea-joliot.png Frédéric Joliot Institute for Life Sciences - On the same theme https://joliot.cea.fr/drf/joliot/en Functionalization of the B subunit of Shiga toxin as a vaccine tool https://www.cea.fr/drf/joliot/en/Pages/news/Science/2024/Functionalization-B-subunit-Shigatoxin-vaccine-tool.aspx Researchers at SIMoS (DMTS) describe the synthesis, engineering and evaluation of the properties of the B subunit of bacterial Shiga toxin (STxB), administered mucosally, as a vaccine tool. Their results confirm the value of using a synthetic STxB in an anti-tumor and anti-infectious vaccination strategy. Biotechnology ; Vaccine SIMOPRO Scientific result préclinique; Fri, 26 Jan 2024 11:00:00 GMT Deciphering microbiota: multiplex proteotyping speeds up identification of microbial isolates https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Multiplex-proteotyping-for-identification-microbial-isolates.aspx Researchers at SPI are proposing an innovative multiplexing method for rapid identification of several microbial isolates in a single mass spectrometry analysis. This approach opens up prospects for the large-scale identification of microorganisms resulting from culturomics programs. High-throughput screening ; Methodologies for life sciences ; Proteomics ; Bacteria SPI Scientific result Fri, 24 Nov 2023 11:00:00 GMT Metaproteomics and data science for an eco-health diagnosis of soil microbiota https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Metaproteomics-data-science-soil-microbiota.aspx Researchers from Li2D (SPI/DMTS, Marcoule) and LSCE have developed an innovative methodology for analyzing soil microbiota based on an optimal computational interpretation of metaproteomic databases, which should lead to a better understanding of the impact of pollutants on soil quality and to the prediction of their restoration potential. Environment ; Proteomics ; Mass spectrometry ; Bacteria Scientific result Thu, 07 Oct 2021 10:00:00 GMT Salmonellosis and shigellosis: a monoclonal antibody induces cross-protection https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Salmonellosis-shigellosis-monoclonal-Ab-cross-protection.aspx Researchers at CEA-Joliot (SPI unit in collaboration with SIMoS unit) have developed a monoclonal antibody directed against a common region of two proteins of the bacteria Shigella and Salmonella and capable of inducing cross-protection against infections caused by these bacteria. Bacteria ; Vaccine Researchers SIMOPRO Scientific result Antibody;IpaD-318;host cell;injectiosome; Thu, 25 Mar 2021 11:00:00 GMT Amazing discovery: Variovorax, a bacterium that lives in the heart of a working nuclear reactor! https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Variovorax-bacteria-living-heart-working-nuclear-reactor.aspx Researchers from I2BC and SPI (DMTS, Marcoule), in collaboration with CEA Grenoble and the Belgian nuclear research center, discovered, by using two meta-omic approaches, living bacteria in the core pool of the CEA Paris-Saclay research reactor, Osiris, in operation, thus opening up perspectives for the discovery of new radioresistant species. Bacteria ; Nuclear Toxicology Scientific result Thu, 17 Dec 2020 11:00:00 GMT Flexizymes help undestanding the functioning of cyclodipeptide synthases https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Flexizymes-undestanding-functioning-cyclodipeptide-synthases.aspx A team from I2BC (SBIGeM), in collaboration with DMTS, took advantage of flexizymes technology to elucidate the functioning of the synthases of cyclodipeptides, enzymes implicated in the synthesis of 2,5-diketopiperazines, compounds that represent an unequaled source of bioactive molecules for the pharmacopoeia. Health & life sciences ; Bacteria Scientific result Thu, 05 Nov 2020 11:00:00 GMT Composition of the microbiota of animals in natura: a challenge met by mass spectrometry https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Composition-microbiota-animals-in-natura-mass-spectro.aspx A team from SPI in Marcoule, in collaboration with INRAE, characterized the intestinal microbiota of a small crustacean, Gammarus fossarum, used as a sentinel of water quality in France, by combining proteogenomics and metaproteomics approaches, thus opening up interesting perspectives for the analysis of host/microbiota interactions in other animal models. Mass spectrometry ; Bacteria ; Metagenomics ; Proteomics Scientific result Mon, 24 Aug 2020 10:00:00 GMT Bacteria and archaea: a new taxonomy to better describe the unknowns? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/New-taxonomy-bacteria-archea.aspx The discovery of myriads of new species of bacteria and archaea, linked to the explosion of metagenomics, is leading microbiologists to consider rethinking how to map certain branches of life. Tools, such as the phylopeptidomics developed at Li2D, could help them do so. Mass spectrometry ; Bacteria ; Proteomics Researchers SPI Mon, 20 Jul 2020 10:00:00 GMT Mapping of Ebola T-cell response: towards a new vaccine ? https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Mapping-Ebola-T-cell-response.aspx SIMoS researchers, in collaboration with Genopole, Excellgene, Vaxeal holding SA (Switzerland), used a large-scale approach to map and characterize the response of CD4 T-cells from healthy donors, against two proteins from the Ebola-Zaire virus. They observed a strong response to the viral nucleoprotein (NP) and suggest that components of NP may be included in the design of new Ebola vaccines. Virus ; Vaccine Scientific result Wed, 24 Jun 2020 10:00:00 GMT Salmonellosis and shigellosis: first demonstration of cross-protection induced by virulence factors SipD and IpaD https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Salmonella-shigella-cross-protection.aspx SPI researchers show for the first time, in a mouse model, the induction of cross-protection against Salmonella and Shigella infections, two pathogenic bacteria responsible for gastrointestinal diseases (typhoid, dysentery among others), one of the main causes of morbidity and mortality in several regions of Africa and Asia. Vaccine ; Bacteria Scientific result Mon, 22 Jun 2020 10:00:00 GMT How the identification of attenuated forms of the SARS-CoV-2 virus could help to control the current epidemic ? https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2020/Identification-attenuated-covid-virus-variants.aspx An opinion article, published in Environmental Microbiology, and written by an international consortium initiated by three researchers of Joliot Institute, proposes an original way of research on Covid-19 through the identification by sequencing of attenuated variants of the SARS-CoV-2 virus in populations at risk with little or no symptoms. Virus ; Genomics ; Vaccine Tue, 12 May 2020 10:00:00 GMT Lassa fever: a vaccine soon to be tested. https://www.cea.fr/drf/joliot/en/Pages/news/Science/2019/Lassa-fever-vaccine.aspx Researchers from Pasteur Institute, in collaboration with seven research teams, including SPI (LI2D/Marcoule), developed and evaluated the efficacy of several vaccine candidates against the virus responsible for Lassa haemorrhagic fever, endemic in Western Africa. They identified one of them as being the most effective, likely to quickly enter clinical trials in humans. Results are published in the journal Science Translational Medicine. Vaccine Scientific result Wed, 09 Oct 2019 10:00:00 GMT Mass spectrometry for quality control of vaccines https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/spectrometry-quality-control-vaccines.aspx ​A SPI Research Team (LI2D, Marcoule), in collaboration with CIRAD and AU-PANVAC, proposes a new quality control technique for veterinary vaccines, based on very high resolution mass spectrometry. This strategy, based on the use of an ad hoc database to measure the proportion of antigens and contaminants in the vaccine preparation, will improve the quality control of vaccines against contagious caprine pleuropneumonia. Mass spectrometry ; Vaccine Scientific result Thu, 05 Jul 2018 10:00:00 GMT Toxins, vaccines, biobetters: a mission at the Butantan Institute of São Paulo https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2018/Toxins-vaccines-biobetters-butantan.aspx ​​Last April, a delegation of experts from the DRF visited colleagues at the Butantan Institute of São Paulo, a biomedical research center that has had close links with several researchers at the Joliot Institute since 2014. This mission is is concluded by the signing of a framework agreement between the two institutes in the field of therapeutic and vaccine innovation. Institutional ; Toxicology ; Vaccine News Fri, 25 May 2018 10:00:00 GMT A trick of nature: a water cluster makes the activation of a DNA photolyase more efficient https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/water-cluster-photolyase.aspx In collaboration with a German Research Team from Marburg University, Pavel Müller and Klaus Brettel (I2BC @ Saclay / SB2SM) have studied the first steps of photoactivation of a Class II photolyase of the Methanosarcina mazei archaeobacteria by resolute optical spectroscopy time and discovered a peculiarity of this class of photolyases: photoinduced separation of charges is stabilized by a water cluster, a structural element also preserved in class II photolyases of plants and animals. Bacteria ; Health & life sciences Scientific result Wed, 14 Feb 2018 11:00:00 GMT A disruptor of late endosomes with anti-infectious properties https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/disruptor-late-endosomes.aspx A SIMOPRO Research Team, in collaboration with a SCBM Team, has identified, by screening a library of small chemical molecules, a compound capable of reducing the sensitivity of cells to a plant toxin, that is ricin. By collaborating with seven Teams of microbiologists, researchers have shown that this compound, called ABMA, protects cells by blocking an intracellular transport pathway borrowed by many other bacterial and intracellular pathogenic toxins. ABMA defines a new family of broad-spectrum anti-infective agents.​ Health & life sciences ; High-throughput screening ; Bacteria Scientific result Tue, 16 Jan 2018 11:00:00 GMT Antibiotic resistance: a Joliot Institute project funded by EIT Health https://www.cea.fr/drf/joliot/en/Pages/news/Science/2017/antibiotic-resistance-a-joliot-institute-project-funded-by-eit-health.aspx ​A SPI/LERI Research Team has recently developed new test strips for the detection of some multi-resistant bacteria. A hospitalization project for these high-speed screening tests will be rolled out in 2018 with funding from EIT Health. Health & life sciences ; Antibiotics ; Bacteria Scientific result Wed, 29 Nov 2017 11:00:00 GMT