https://joliot.cea.fr/drf/joliot/en/Pages/news/Science/2020/Composition-microbiota-animals-in-natura-mass-spectro.aspx Website of the Frédéric-Joliot Institute of Life Sciences which includes 4 departments: I2BC, DMTS, NeuroSpin and Service hospitalier Frédéric Joliot (SHFJ). The teams are mainly located at the CEA Paris-Saclay center and at the Orsay hospital. en-US https://www.cea.fr/Style%20Library/Themes/drf/instituts/images/logo-cea-joliot-2023.png https://joliot.cea.fr/drf/joliot/en https://www.cea.fr/drf/joliot/en/Pages/news/Science/2026/Chemical-probes-profiling-active-metalloproteases.aspx Researchers from SIMoS, in collaboration with the University of Montpellier, have designed a highly selective chemical probe targeting the active form of extracellular matrix metalloproteases, with the aim of enabling single-step multiplex profiling by mass spectrometry within complex proteomes. Chemistry ; Biotechnology ; Mass spectrometry SIMOPRO Scientific result ; Headlines Fri, 21 Aug 2026 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2026/TSPO-key-protein-porphyrin-iron-metabolism-Bcereus.aspx Researchers from Avignon University and SPI have investigated the proteome of the bacterium Bacillus cereus grown under anoxic conditions in order to gain a better understanding of the role of the TSPO protein in the bacterium’s physiology. They demonstrate that TSPO is a key regulator of porphyrin and iron metabolism, helping to maintain the environmental resilience of B. cereus. Proteomics ; Environment ; Mass spectrometry ; Bacteria SPI Scientific result Fri, 03 Jul 2026 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2026/Biomarkers-hypoxic-brain-injury-choroid-plexus-organoids.aspx A team from the SPI (DMTS) investigated the effects of hypoxia on the interface between the choroid plexus and cerebrospinal fluid using organoids derived from human pluripotent stem cells. The aim was to investigate how cerebral hypoxia at birth, which causes hypoxic-ischaemic encephalopathy, disrupts this cerebral barrier, which has not yet been extensively studied. Brain ; Diseases ; Proteomics SPI Scientific result ; Article Fri, 17 Apr 2026 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2026/spectrometrie-masse-exposomes.aspx Researchers from the DMTS have developed a method for exploring the chemical exposome in large, complex mass spectrometry datasets. They applied it to the analysis of meconium samples from a cohort. Mass spectrometry Researchers SPI Scientific result Thu, 26 Feb 2026 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2026/Analysis-global-metabolism-carbon-13-isotopic-labeling.aspx A collaborative study between the BIAM and Joliot (SPI/DMTS) institutes demonstrates the feasibility of near-complete whole-body carbon-13 isotopic labeling in mice and lays the foundation for a global, quantitative, and unbiased metabolomics approach. This unprecedented strategy could also enable the investigation of certain metabolic troubles. Mass spectrometry ; Metabolomics BIAM Scientific result Mon, 09 Feb 2026 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2025/mineMS2.aspx A LI-MS team presents an innovative strategy and software for structural elucidation in metabolomics using tandem mass spectrometry. Mass spectrometry ; Metabolomics Researchers SPI Article ; Scientific result Fri, 07 Nov 2025 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2025/alpha-synucleine-formes-tronquees-MS.aspx A team from LI-MS (SPI/DMTS) combines a robust α-synuclein immunoprecipitation protocol with high-resolution mass spectrometry and characterizes novel truncated forms of the protein. Proteomics ; Biomarkers Researchers SPI Scientific result ; Article Mon, 20 Oct 2025 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2025/COVID-PATH.aspx ​​The COVID-PATH project was officially launched on March 12, 2025, to investigate the gastrointestinal impact of SARS-CoV-2 and its link to Long COVID​. Infectious diseases ; Proteomics ; Metagenomics ; Virus Researchers SPI Europe ; Academic partnerships ; Article Thu, 10 Apr 2025 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2025/Universal-identification-pathogenic-viruses-proteotyping.aspx Researchers at Li2D (SPI/DMTS, Marcoule) have proposed an innovative tandem mass spectrometry proteotyping method for the universal identification of pathogenic viruses, requiring no prior knowledge of sample composition. A step towards improved diagnosis of viral infections Virus ; Mass spectrometry ; Biodetection ; CBRN-E threats SPI Scientific result ; Article Wed, 29 Jan 2025 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2025/Cancer-immunoprofiling-click-chemistry-mass-spectrometry.aspx Researchers at DMTS and SHFJ have carried out a multiplexed analysis of tumor cells and tissues, based on the use of cleavable therapeutic antibodies carrying mass tags, for the identification and quantification of cancer biomarkers. A proof-of-concept demonstrating the high potential of this tumor immunoprofiling approach Cancer ; Chemistry ; Mass spectrometry ; Diagnosis SIMOPRO Scientific result preclinical research;animal models; Mon, 13 Jan 2025 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2024/Diagnosis-acute-hepatic-porphyrias-mass-spectrometry.aspx A collaborative study between SPI and a team from Hôpital Louis Mourier demonstrates the contribution of liquid chromatography coupled with mass spectrometry to the day-to-day analytical needs of a clinical laboratory. Application to the rapid quantification of biomarkers in acute hepatic porphyrias. Diagnosis ; Mass spectrometry ; Biomarkers SPI Scientific result ; Article Fri, 23 Feb 2024 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Multiplex-proteotyping-for-identification-microbial-isolates.aspx Researchers at SPI are proposing an innovative multiplexing method for rapid identification of several microbial isolates in a single mass spectrometry analysis. This approach opens up prospects for the large-scale identification of microorganisms resulting from culturomics programs. High-throughput screening ; Methodologies for life sciences ; Proteomics ; Bacteria SPI Scientific result Fri, 24 Nov 2023 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Proteins-involved-pathogenesis-creatine-transporter-deficiency.aspx Researchers at SPI (DMTS) have identified by mass spectrometry and bioinformatics analysis several biomarkers linked to the creatine transporter deficiency disorder (CTD) using two original models : mice with the clinical characteristics of CTD subjects and brain organoids derived from CTD patient cells. Mass spectrometry ; Genetic diseases ; Biomarkers ; Proteomics SPI Scientific result Thu, 22 Jun 2023 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Identification-cystic-fibrosis-lung-microbiota-metaproteomics.aspx A Li2D (SPI/DMTS) team used tandem mass spectrometry to identify microorganisms in sputum samples from cystic fibrosis patients. A promising proteotyping approach that provides an expanded and unbiased view of the cystic fibrosis microbiota and could be complementary to microbiological monitoring of patients. Large-scale biology ; Methodologies for life sciences ; Proteomics SPI Scientific result Tue, 14 Feb 2023 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2023/Cone-marine-toxin-melanocortin-receptor-antagonist.aspx A SIMoS team discovered a toxin in the venom of a marine cone that it engineers into a high affinity antagonistic peptide for the melanocortin receptor, a major regulator of energy homeostasis. A step towards the development of innovative treatments for certain eating disorders Pharmacology ; High-throughput screening ; Proteomics ; Transcriptomics SIMOPRO Scientific result Thu, 09 Feb 2023 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Link-intestinal-microbiota-Covid-19.aspx A team from SPI used mass spectrometry to analyze the degree of alteration of the intestinal microbiota of Covid-19 patients and to look for signatures of intestinal infection by the SARS-CoV-2 virus. The level of the observed gut dysbiosis would be an indicator of the stage and extent of the infection. Mass spectrometry ; Proteomics ; Biomarkers ; Infectious diseases SPI Scientific result ; Article Thu, 12 May 2022 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/ProMetIS-open-database-multi-omics-phenotyping.aspx A consortium of researchers, led by SPI/DMTS, presents ProMetIS, a pilot study for the deep phenotyping of murine models by combining proteomic and metabolomic approaches. A significant advance for the functional characterization of genes and the development of bioinformatics integration approaches. Mass spectrometry ; Large-scale biology ; Bioinformatic SPI Scientific result Mon, 04 Apr 2022 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2022/Diagnosis-MS-congenital-disorders-glycosylation.aspx A collaboration initiated in 2017 between AP-HP (Bichat Hospital), Paris-Saclay University (Inserm 1193) and the Laboratoire d’Études du Métabolisme des Médicaments (LEMM/SPI/DMTS), leading to 4 publications in 2021, is advancing knowledge on the origin of certain congenital disorders of glycosylation, through the identification of specific biochemical biomarkers. Mass spectrometry ; Biomarkers ; Genetic diseases SPI Scientific result Tue, 04 Jan 2022 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Metaproteomics-data-science-soil-microbiota.aspx Researchers from Li2D (SPI/DMTS, Marcoule) and LSCE have developed an innovative methodology for analyzing soil microbiota based on an optimal computational interpretation of metaproteomic databases, which should lead to a better understanding of the impact of pollutants on soil quality and to the prediction of their restoration potential. Environment ; Proteomics ; Mass spectrometry ; Bacteria Scientific result Thu, 07 Oct 2021 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Salmonellosis-shigellosis-monoclonal-Ab-cross-protection.aspx Researchers at CEA-Joliot (SPI unit in collaboration with SIMoS unit) have developed a monoclonal antibody directed against a common region of two proteins of the bacteria Shigella and Salmonella and capable of inducing cross-protection against infections caused by these bacteria. Bacteria ; Vaccine Researchers SIMOPRO Scientific result Antibody;IpaD-318;host cell;injectiosome; Thu, 25 Mar 2021 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Food-poisoning-SE-targeted-mass-spectrometry.aspx SPI (DMTS), in collaboration with ANSES, has developed a test based on immuno-capture and targeted mass spectrometry for the quantitative and multiplex identification, in dairy products, of the eight main enterotoxins of Staphylococcus aureus, responsible for food poisoning epidemics. Mass spectrometry ; Toxicology ; CBRN-E threats Scientific result Thu, 18 Mar 2021 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2021/Vers-test-quantitatif-MS-diagnostic-specifique-COVID-19.aspx Le LEMM, en collaboration avec le LI2D et l’AP-HP, développe un test de diagnostic spécifique de la Covid-19 par spectrométrie de masse qui permettrait aussi la quantification de la charge virale du patient. Objectif à terme : adapter la méthode pour la rendre plus sensible et compatible avec les équipements d’analyse des hôpitaux. Diagnosis ; Mass spectrometry ; Virus Researchers SPI Scientific result SARS-CoV-2; Tue, 09 Mar 2021 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Institute/2021/SPI-ATRACTion-consortium.aspx Coordinated by the Imagine Institute for Genetic Diseases, the ATRACTion program is based on a consortium of 11 academic and industrial partners, including SPI (DMTS), and aims to develop a precision medicine for patients with autoimmune and inflammatory diseases associated with primary immune deficiencies. Metabolomics ; Mass spectrometry ; Autoimmune diseases News Thu, 14 Jan 2021 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Variovorax-bacteria-living-heart-working-nuclear-reactor.aspx Researchers from I2BC and SPI (DMTS, Marcoule), in collaboration with CEA Grenoble and the Belgian nuclear research center, discovered, by using two meta-omic approaches, living bacteria in the core pool of the CEA Paris-Saclay research reactor, Osiris, in operation, thus opening up perspectives for the discovery of new radioresistant species. Bacteria ; Nuclear Toxicology Scientific result Thu, 17 Dec 2020 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Flexizymes-undestanding-functioning-cyclodipeptide-synthases.aspx A team from I2BC (SBIGeM), in collaboration with DMTS, took advantage of flexizymes technology to elucidate the functioning of the synthases of cyclodipeptides, enzymes implicated in the synthesis of 2,5-diketopiperazines, compounds that represent an unequaled source of bioactive molecules for the pharmacopoeia. Health ＆ life sciences ; Bacteria Scientific result Thu, 05 Nov 2020 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Composition-microbiota-animals-in-natura-mass-spectro.aspx A team from SPI in Marcoule, in collaboration with INRAE, characterized the intestinal microbiota of a small crustacean, Gammarus fossarum, used as a sentinel of water quality in France, by combining proteogenomics and metaproteomics approaches, thus opening up interesting perspectives for the analysis of host/microbiota interactions in other animal models. Mass spectrometry ; Bacteria ; Metagenomics ; Proteomics Scientific result Mon, 24 Aug 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/SARS-CoV-2-detection-mass-spectrometry-rapidity.aspx A team from SPI in Marcoule detected by mass spectrometry, in 3 minutes and without specific reagents, signature peptides of the SARS-CoV-2 virus in clinical samples (nasopharyngeal swabs). It thus provides a proof of concept of the use of this method as a possible alternative to PCR, currently the reference method. Mass spectrometry ; Virus ; Biodetection ; Diagnosis Scientific result Thu, 06 Aug 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Venomics-2-toxines-MC4R.aspx In an article published in the Journal of Medicinal Chemistry, the Venomics consortium led by a SIMoS team shows the interest of creating toxin banks to identify molecules regulating therapeutic targets. High-throughput screening ; Proteomics ; Transcriptomics SIMOPRO Scientific result Thu, 23 Jul 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/New-taxonomy-bacteria-archea.aspx The discovery of myriads of new species of bacteria and archaea, linked to the explosion of metagenomics, is leading microbiologists to consider rethinking how to map certain branches of life. Tools, such as the phylopeptidomics developed at Li2D, could help them do so. Mass spectrometry ; Bacteria ; Proteomics Researchers SPI Mon, 20 Jul 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Proteomic-analysis-SARS-CoV-2-infected-cells.aspx In order to optimize the amplification of quality inactivated virus, for the manufacture of a vaccine, a team from SPI (CEA Marcoule) used mass spectrometry to analyze the dynamics of the proteome of SARS-CoV-2 infected cells, at two multiplicities of infection. With more than 3,220 identified host proteins, researchers are also starting to decipher the processes and cellular networks impacted by this virus. Virus ; Mass spectrometry ; Proteomics News Mon, 06 Jul 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Salmonella-shigella-cross-protection.aspx SPI researchers show for the first time, in a mouse model, the induction of cross-protection against Salmonella and Shigella infections, two pathogenic bacteria responsible for gastrointestinal diseases (typhoid, dysentery among others), one of the main causes of morbidity and mortality in several regions of Africa and Asia. Vaccine ; Bacteria Scientific result Mon, 22 Jun 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Proteomics-SARS-CoV-2-signature-peptides.aspx SPI researchers (Marcoule) used proteomics to identify signature peptides of the SARS-CoV-2 virus expressed in vitro. A "short list" of 14 identified and characterized peptides allows to consider developments in targeted mass spectrometry, making this direct and rapid large-scale approach, implantable in hospitals, a potential tool of choice in the detection of the virus responsible for Covid-19. Proteomics ; Virus Scientific result Fri, 05 Jun 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Phylopeptidomics-analysis-microbiota.aspx ​Researchers at the "Laboratoire Innovations technologiques pour la Détection et le Diagnostic" (LI2D, in Marcoule) of the Department of Medicines and Technologies for Health (DMTS) have developed a mathematical method to identify in a metaproteomics dataset the contribution of each of the organisms that make up a microbiota. This robust method, called phylopeptidomics, is described in the journal Microbiome. Proteomics ; Biodiversity SPI Scientific result Microbiology; Mon, 06 Apr 2020 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2020/Characterization-predictive-survival-biomarker-septic-shock.aspx Two recent studies conducted by SPI researchers, in collaboration with Inserm and AP/HP teams, reveal the value of measuring plasma levels of calgranulins as biomarkers for the prediction of mortality risk of patients with septic shock, admitted in intensive care unit. Biomarkers ; Mass spectrometry ; Infectious diseases Scientific result Tue, 24 Mar 2020 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2019/Alzheimer-or-Lewy-bodies-dementia-MS-answer.aspx A team from SPI, in collaboration with the metabolic biochemistry service and the Institute of Memory and Alzheimer's Disease (IM²A) of the university hospitals Pitié-Salpêtrière, has developed a method for the simultaneous quantification of tau and α-synuclein proteins in human cerebrospinal fluid by high-resolution mass spectrometry. A promising new approach for the differential diagnosis of Lewy bodies dementia and Alzheimer's disease. Neurodegenerative diseases ; Mass spectrometry Scientific result Thu, 07 Nov 2019 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/new-protein-interface.aspx ​A SB2SM Research Team (I2BC@Saclay) solved by crystallography the structure of a ternary protein complex at the interface between the nuclear envelope (emerin and lamin A / C) and the genome (BAF). In collaboration with Teams from Paris-Sud and Paris-Diderot Universities, the structure analysis, as well as in vitro interaction and proximity experiments in cellulo, suggests that a lack of interaction between the lamina A / C (nucleoskeleton) and chromatin-associated protein (BAF) may be one of the mechanisms responsible for premature aging syndromes. Proteomics Scientific result Thu, 20 Sep 2018 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/spectrometry-quality-control-vaccines.aspx ​A SPI Research Team (LI2D, Marcoule), in collaboration with CIRAD and AU-PANVAC, proposes a new quality control technique for veterinary vaccines, based on very high resolution mass spectrometry. This strategy, based on the use of an ad hoc database to measure the proportion of antigens and contaminants in the vaccine preparation, will improve the quality control of vaccines against contagious caprine pleuropneumonia. Mass spectrometry ; Vaccine Scientific result Thu, 05 Jul 2018 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/server-predict-structure-protein-complexes.aspx A team from I2BC@Saclay (SB2SM), in collaboration with the RPBS platform, has put online a server for structural prediction of interactions between proteins. This server, designed for biologists, uses the information provided by the coevolution of protein sequences and predicts the structure of protein complexes, essential information for understanding their functional role. Proteomics Scientific result Thu, 07 Jun 2018 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/metabolomic-analysis-facilitated-by-mass-spectrometry.aspx In a study published in Analytical Chemistry, the Mass Spectrometry Research Team of the Laboratory Drug Metabolism Research Laboratory (SPI) demonstrated the interest of Orbitrap Fusion, a state-of-the-art mass spectrometer for the identification of relevant metabolites based on their masses and isotopes measured with high accuracy. Chemistry ; Metabolomics ; Mass spectrometry Scientific result Wed, 25 Apr 2018 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/ghosts-of-fluorescence.aspx Cell biology uses fluorescent labeling, an essential technique to follow the life of the cell. But fluorescent proteins have their limits, which researchers from IBS and I2BC @ Saclay (SB2SM) are trying to repel. Structural biology ; Proteomics Scientific result Wed, 28 Mar 2018 10:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/water-cluster-photolyase.aspx In collaboration with a German Research Team from Marburg University, Pavel Müller and Klaus Brettel (I2BC @ Saclay / SB2SM) have studied the first steps of photoactivation of a Class II photolyase of the Methanosarcina mazei archaeobacteria by resolute optical spectroscopy time and discovered a peculiarity of this class of photolyases: photoinduced separation of charges is stabilized by a water cluster, a structural element also preserved in class II photolyases of plants and animals. Bacteria ; Health ＆ life sciences Scientific result Wed, 14 Feb 2018 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2018/disruptor-late-endosomes.aspx A SIMOPRO Research Team, in collaboration with a SCBM Team, has identified, by screening a library of small chemical molecules, a compound capable of reducing the sensitivity of cells to a plant toxin, that is ricin. By collaborating with seven Teams of microbiologists, researchers have shown that this compound, called ABMA, protects cells by blocking an intracellular transport pathway borrowed by many other bacterial and intracellular pathogenic toxins. ABMA defines a new family of broad-spectrum anti-infective agents.​ Health ＆ life sciences ; High-throughput screening ; Bacteria Scientific result Tue, 16 Jan 2018 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2017/antibiotic-resistance-a-joliot-institute-project-funded-by-eit-health.aspx ​A SPI/LERI Research Team has recently developed new test strips for the detection of some multi-resistant bacteria. A hospitalization project for these high-speed screening tests will be rolled out in 2018 with funding from EIT Health. Health ＆ life sciences ; Antibiotics ; Bacteria Scientific result Wed, 29 Nov 2017 11:00:00 GMT https://www.cea.fr/drf/joliot/en/Pages/news/Science/2017/Protein_interactome_of_nanoparticles_and_its_role_in_cellular_impact.aspx Two Research Teams led by Odette Prat of the Institute of Biosciences and Biotechnologies (BIAM) and Jean Armengaud of the Frédéric Joliot Institute (LI2D, Marcoule) have joined forces thanks to an ANR funding to deepen knowledge of the importance of the nature of protein crowns on the cellular impact of nanoparticles. The results from their three recent publications reveal a new concept: "the protein interactome applied to the corona of nanoparticles". Health ＆ life sciences ; Proteomics ; Nanosciences Scientific result Fri, 20 Oct 2017 10:00:00 GMT