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Pharmacology and Immunoanalysis unit

Published on 15 November 2018
For 30 years, the Pharmacology and Immunoanalysis (SPI) unit has been demonstrating its expertise in the development of immunoanalytical methods, physicochemical analyzes (especially LC-MS) and cell culture enabling it to address multiple issues concerning detection, quantification and future development of molecules of various and varied kinds.


Head of Unit
Christophe Junot
01 69 08  43 66

Stéphanie Simon
33 1 69 08 77 04


Laurie MENEZ
01 69 08 51 39

Sandrine LEBLOIS
01 69 08 72 99

Stéphanie NANO
01 69 08 45 83

The research carried out in SPI always connects with the fundamental aspects of the methodological and analytical developments and always integrates the "Therapeutic Innovation and Diagnostic" line of the CEA's Fundamental Research Division. Research is usually carried out in collaboration with academic partners or contract with industry or government partners.

The themes developed by the Drug Metabolism Research Laboratory  (LEMM) rely on a large fleet of mass spectrometers and revolve around:
  • the development of new methods of detection, identification and quantification of drugs and their metabolites, as well as recombinant proteins or pathogens,
  • the development of metabolomic, lipidomic and glycomic approaches in the search for diagnostic biomarkers or pathologies monitoring,
  • the development of cell models (in particular an in vitro model of blood brain barrier) for neurovascular pharmacology
  • the engineering of antibodies for therapy and diagnostic by imaging
The subjects of the Immunoanalysis Studies and Research Laboratory (LERI) all involve the "antibody molecule" through the means of production of polyclonal and monoclonal antibodies at its disposal to develop:
  • diagnostic methods (ELISA and immuno-chromatographic tests) for various types of targets: xenobiotics, proteins and especially pathogens handled in the laboratory L3 available at LERI,
  • new technological developments around the "fast" dosages like immunochromatography, 
  • immunotherapeutic or vaccine approaches

The mission of the Technological Innovations for Detection and Diagnosis Laboratory (LI2D), based on the CEA site of Marcoule, is to develop methodologies and technologies for the detection of pathogens or toxic substances found in the environment or in biological samples / tissues and the discovery and validation of biomarkers for diagnosis. 
The research carried out at Li2D is based on his expert knowledge in microbiological, immunological, genetic, genomic, proteomic, bioinformatics and high-resolution mass spectrometry techniques. 

The work done at the Food Immuno-Allergy Unit (UIAA) aims to develop tools and knowledge to assess, manage and if possible prevent food-related allergic risk.

Indeed, food allergies are a major public health problem because of the increase in prevalence, but also the number of affected food and the severity of clinical symptoms they cause. Therefore, it is a challenge and a concern for the Food Industry and the Public Authorities in charge of the health of consumers to have means to assess, manage and if possible prevent the allergic risks of food.

Finally, since its inception, the SPI has developed commercialization projects that proved particularly successful (Prion or bioterrorism agents dosage, for example), by the realization of numerous contractual studies or collaborative research with industrial partners in pharmaceuticals and diagnostics for over twenty years ("spin-off" from our developments).The existence of a strong quality policy also contributes to the identity of our service: the presence of two full-time quality professionals, accreditation of Good Laboratory Practices (ANSM) of LEMM and iso9001 certification of SPI obtained on December 2014 (LI2D in 2003).


Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow's milk
Adel-Patient K., Lezmi G., Castelli F. A., Blanc S., Bernard H., Soulaines P., Dumond P., Ah-Leung S., Lageix F., de Boissieu D., Cortes-Perez N., Hazebrouck S., Fenaille F., Junot C. and Dupont C.
Evaluation of the High-Field Orbitrap Fusion for Compound Annotation in Metabolomics
Barbier Saint Hilaire P., Hohenester U. M., Colsch B., Tabet J. C., Junot C. and Fenaille F.
On-a-chip tryptic digestion of transthyretin: a step toward an integrated microfluidic system for the follow-up of familial transthyretin amyloidosis
Bataille J., Viode A., Pereiro I., Lafleur J. P., Varenne F., Descroix S., Becher F., Kutter J. P., Roesch C., Pous C., Taverna M., Pallandre A., Smadja C. and Le Potier I.
Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation
Benard C., Acket S., Rossez Y., Fernandez O., Berton T., Gibon Y. and Cabasson C.
Immune components of early breastmilk: association with maternal factors and with reported food allergy in childhood
Berdi M., de Lauzon-Guillain B., Forhan A., Castelli F. A., Fenaille F., Charles M. A., Heude B., Junot C. and Adel-Patient K.
Methotrexate and BAFF interaction prevents immunization against TNF inhibitors
Bitoun S., Nocturne G., Ly B., Krzysiek R., Roques P., Pruvost A., Paoletti A., Pascaud J., Donnes P., Florence K., Gleizes A., Hincelin-Mery A., Allez M., Hacein-Bey-Abina S., Mackay F., Pallardy M., Le Grand R. and Mariette X.
A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing Enterobacteriaceae
Boutal H., Vogel A., Bernabeu S., Devilliers K., Creton E., Cotellon G., Plaisance M., Oueslati S., Dortet L., Jousset A., Simon S., Naas T. and Volland H.
Inhibition of Lysosome Membrane Recycling Causes Accumulation of Gangliosides that Contribute to Neurodegeneration
Boutry M., Branchu J., Lustremant C., Pujol C., Pernelle J., Matusiak R., Seyer A., Poirel M., Chu-Van E., Pierga A., Dobrenis K., Puech J. P., Caillaud C., Durr A., Brice A., Colsch B., Mochel F., El Hachimi K. H., Stevanin G. and Darios F.
Inhibition of central de novo ceramide synthesis restores insulin signaling in hypothalamus and enhances beta-cell function of obese Zucker rats
Campana M., Bellini L., Rouch C., Rachdi L., Coant N., Butin N., Bandet C. L., Philippe E., Meneyrol K., Kassis N., Dairou J., Hajduch E., Colsch B., Magnan C. and Le Stunff H.
A novel subfamily of bacterial AAT-fold basic amino acid decarboxylases and functional characterization of its first representative: Pseudomonas aeruginosa LdcA
Carriel D., Garcia P. S., Castelli F., Lamourette P., Fenaille F., Brochier-Armanet C., Elsen S. and Gutsche I.
Orchestration of Tryptophan-Kynurenine pathway, acute decompensation and acute-on-chronic liver failure in cirrhosis
Claria J., Moreau R., Fenaille F., Amoros A., Junot C., Gronbaek H., Coenraad M. J., Pruvost A., Ghettas A., Chu-Van E., Lopez-Vicario C., Oettl K., Caraceni P., Alessandria C., Trebicka J., Pavesi M., Deulofeu C., Albillos A., Gustot T., Welzel T. M., Fernandez J., Stauber R. E., Saliba F., Butin N., Colsch B., Moreno C., Durand F., Nevens F., Banares R., Benten D., Gines P., Gerbes A., Jalan R., Angeli P., Bernardi M. and Arroyo V.
Targeted versus untargeted omics - the CAFSA story
Del Mar Amador M., Colsch B., Lamari F., Jardel C., Ichou F., Rastetter A., Sedel F., Jourdan F., Frainay C., Wevers R. A., Roze E., Depienne C., Junot C. and Mochel F.
Ducancel F., Delache B., Navarro F., Viargues P., Dano J., Contreras V., Gillet D., Barbier J. and Le Grand, R.
MetaboRank: network-based recommendation system to interpret and enrich metabolomics results
Frainay C., Aros S., Chazalviel M., Garcia T., Vinson F., Weiss N., Colsch B., Sedel F., Thabut D., Junot C. and Jourdan F.
Proteogenomic insights into uranium tolerance of a Chernobyl's Microbacterium bacterial isolate
Gallois N., Alpha-Bazin B., Ortet P., Barakat M., Piette L., Long J., Berthomieu C., Armengaud J. and Chapon V.
The PEG-responding desiccome of the alder microsymbiont Frankia alni
Ghedira K., Harigua-Souiai E., Ben Hamda C., Fournier P., Pujic P., Guesmi S., Guizani I., Miotello G., Armengaud J., Normand P. and Sghaier H.
CCDC115-CDG: A new rare and misleading inherited cause of liver disease
Girard M., Poujois A., Fabre M., Lacaille F., Debray D., Rio M., Fenaille F., Cholet S., Ruel C., Causse E., Selves J., Bridoux-Henno L., Woimant F., Dupre T., Vuillaumier-Barrot S., Seta N., Alric L., de Lonlay P. and Bruneel A.
Ecotoxicoproteomics: A decade of progress in our understanding of anthropogenic impact on the environment
Gouveia D., Almunia C., Cogne Y., Pible O., Degli-Esposti D., Salvador A., Cristobal S., Sheehan D., Chaumot A., Geffard O. and Armengaud J.
Identification, expression, and endocrine-disruption of three ecdysone-responsive genes in the sentinel species Gammarus fossarum
Gouveia D., Bonneton F., Almunia C., Armengaud J., Queau H., Degli-Esposti D., Geffard O. and Chaumot A.
Impact of Cross-reactivity between Major Peanut Allergens Ara h 2 and Ara h 6 on Specific IgE Measurements
Guillon B., Bernard H., Paty E., Dreskin S. C., Adel-Patient K. and Hazebrouck S.
Journal of Allergy and Clinical Immunology 141 (2), AB237-AB237, 2018
Conformational IgE-binding Epitopes of Major Peanut Allergen Ara h 2 Revealed with Chimeric 2S-albumins
Hazebrouck S., Guillon B., Paty E., Adel-Patient K. and Bernard H.
Journal of Allergy and Clinical Immunology 141 (2), AB179-AB179, 2018
Antibodies targeting human endothelin-1 receptors reveal different conformational states in cancer cells
Herbet A., Costa N., Leventoux N., Mabondzo A., Couraud J. Y., Borrull A., Hugnot J. P. and Boquet D.
Physiol Res 67 (Supplementum 1), S257-s264, 2018
Evaluation of the NG-Test CARBA 5 multiplex immunochromatographic assay for the detection of KPC, OXA-48-like, NDM, VIM and IMP carbapenemases
Hopkins K. L., Meunier D., Naas T., Volland H. and Woodford N.
The anti-caspase inhibitor Q-VD-OPH prevents AIDS disease progression in SIV-infected rhesus macaques
Laforge M., Silvestre R., Rodrigues V., Garibal J., Campillo-Gimenez L., Mouhamad S., Monceaux V., Cumont M. C., Rabezanahary H., Pruvost A., Cordeiro-da-Silva A., Hurtrel B., Silvestri G., Senik A. and Estaquier J.
Time-course proteomics dataset to monitor protein-bound methionine oxidation in Bacillus cereus ATCC 14579
Madeira J. P., Alpha-Bazin B., Armengaud J. and Duport C.
Assessing the ratio of Bacillus spores and vegetative cells by shotgun proteomics
Mappa C., Pible O., Armengaud J. and Alpha-Bazin B.
Baseline urine metabolic phenotype in patients with severe alcoholic hepatitis and its association with outcome
Maras J. S., Das S., Sharma S., Shasthry S. M., Colsch B., Junot C., Moreau R. and Sarin S. K.
Importance of Post-translational Modifications in the Interaction of Proteins with Mineral Surfaces: The Case of Arginine Methylation and Silica surfaces
Marichal L., Renault J. P., Chedin S., Lagniel G., Klein G., Aude J. C., Tellier-Lebegue C., Armengaud J., Pin S., Labarre J. and Boulard Y.
A fluorine scan of a tubulin polymerization inhibitor isocombretastatin A-4: Design, synthesis, molecular modelling, and biological evaluation
Naret T., Bignon J., Bernadat G., Benchekroun M., Levaique H., Lenoir C., Dubois J., Pruvost A., Saller F., Borgel D., Manoury B., Leblais V., Darrigrand R., Apcher S., Brion J. D., Schmitt E., Leroux F. R., Alami M. and Hamze A.
Investigating the utility of minimized sample preparation and high-resolution mass spectrometry for quantification of monoclonal antibody drugs
Nguyen Tttn, Mistarz U. H., Costa N., Herbet A., Boquet D., Becher F. and Rand K. D.
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