To carry out their activities, Research Teams of the Frédéric Joliot Institute for Life Sciences have developed high-profile technological platforms in many areas : biomedical imaging, structural biology, metabolomics, High-Throughput screening, level 3 microbiological safety laboratory...
Within the Institute, the "Funding Research and Technology Transfer" team is at your disposal to identify the scientists and the skills you need to set up a joint project, to define the terms of a collaboration contract or study.
Whether you are an academic, a SME or an industrialist, our team informs and advices you about the possibilities of consortium assembly, technology transfer, patent licensing or use of our platforms.
The team is also at the disposal of the researchers of the institute to accompany them in achieving their valorization objectives.
All the news of the Institute of life sciences Frédéric Joliot
Scientific result | Mass spectrometry | Bacteria | Metagenomics | Proteomics
A team from SPI in Marcoule, in collaboration with INRAE, characterized the intestinal microbiota of a small crustacean, Gammarus fossarum, used as a sentinel of water quality in France, by combining proteogenomics and metaproteomics approaches, thus opening up interesting perspectives for the analysis of host/microbiota interactions in other animal models.
In a healthy state, microbiota (ensemble of microorganisms - bacteria, archaea, fungi, and viruses -and their collective genomes, termed microbiome), can mediate a variety of functions and biological processes in their hosts such as nutrition, energy metabolism, immune responses, or neurotransmission. Among the approaches available for studying microbiomes, metaproteomics, defined as the large-scale identification and quantification of proteins from microbial communities, is rapidly gaining traction as a method to directly observe the protein complement of organisms. Due to the paucity of molecular information for numerous animal species such as arthropods, microbial communities from these animals remain poorly characterized. Animal metaproteomics have focused on exploring the microbiomes from large animals such as mammals or birds and studies on small invertebrates are scarce. Metaproteomic data may be assigned using generalist databases encompassing all known genomes. In the present study, researchers from SPI in Marcoule (Li2D) propose an iterative generalist database search strategy combining high-resolution mass spectrometry of proteins and RNA-seq of the host to characterize the microbiome of an organism not yet genome-sequenced. They propose to complement generalist databases with an RNA-seq-based protein database specific for the host, by using a previously RNA-seq data set acquired on Gammarus fossarum. The workflow was applied for the study of the microbiome of two tissues from the digestive tract of G. fossarum, a key species for aquatic systems with a body length of 2 to 15 mm, and an emergent model in environmental toxicology.
Gammarus fossarum © J.Armengaud/CEA
The pipeline proposed in this study allows discriminating host, residual food, and microbiome proteins, and identifies the main taxa and their functions at an unprecedented range, including archaea, bacteria, fungi, and parasites. These approaches pave the way for performing deep mechanistic studies in both host and host-associated microorganisms, and for fuller understanding of microbiome–host relationships.
D. Gouveia, O Pible, K Culotta, V. Jouffret, O. Geffard, A. Chaumot, D. Degli-Esposti, J. Armengaud Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host (2020) NPG Biofilms & Microbiomes, 6(1):23. https://www.nature.com/articles/s41522-020-0133-2
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.